Thermo Fisher Scientific HSP90 Monoclonal Antibody (H9010), PerCP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,500

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:100,000

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Antibody Microarray (AM)

Assay-dependent

-

Product Specifications

Species Reactivity

Dog, Chicken, Fish, Hamster, Human, Mouse, Rabbit, Rat, Shark

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

H9010

Immunogen

Recombinant human HSP90beta

Conjugate

PerCP PerCP PerCP

View additional formats

• APC
• FITC
• Request custom conjugation

Excitation/Emission Max

482/675 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

95.46mM phosphate/2.48mM MES, pH 7.2, with 2mM EDTA

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931558

Product Specific Information

1 µg/mL of MA5-45104 was sufficient for detection of HSP90beta in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects 90kDa. Detects HSP90 beta in all reactive species except in Chicken, where it detects both alpha and beta isoforms.

Target Information

HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1, an inducible form, and HSP90AB1 and mitochondria (Chen et al., 2005 ).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.