Thermo Fisher Scientific HSP70/HSC70 Monoclonal Antibody (BB70), APC
HSP70/HSC70 단백질을 검출하기 위한 APC 결합 단일클론 항체입니다. Western blot, IHC, ICC/IF, IP 등 다양한 응용에 적합합니다. 광범위한 종에 반응하며, 단백질 G로 정제된 고순도 항체입니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Dog, Chicken, Fruit fly, Fish, Guinea pig, Hamster, Human, Mammal, Mouse, Sheep, Pig, Rabbit, Rat, Xenopus, Yeast |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BB70 |
| Immunogen | Chicken HSP70/HSP90 complex |
| Conjugate | APC |
| Excitation/Emission Max | 651/660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.2, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931553 |
Available Formats
- FITC (Catalog No. MA5-45100)
- PerCP (Catalog No. MA5-45101)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45099 was sufficient for detection of HSP70 and HSC70 in 20 µg of heat-shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 72 kDa (HSP) and approximately 73 kDa (HSC).
Target Information
HSPA4 (Heat Shock Protein Family A (Hsp70) Member 4) is a protein-coding gene associated with Chagas Disease and Babesiosis. Related pathways include regulation of activated PAK-2p34 by proteasome-mediated degradation and cellular responses to stimuli.
An important paralog of this gene is HSPH1.
HSPA8 (Heat Shock Protein Family A (Hsp70) Member 8) is associated with auditory system disease and Borna disease. Related pathways include processing of capped intron-containing pre-mRNA and selective autophagy.
Gene Ontology (GO) annotations include RNA binding and ubiquitin protein ligase binding.
An important paralog of this gene is HSPA2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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