Thermo Fisher Scientific HSP70/HSC70 Monoclonal Antibody (BB70), PerCP
HSP70/HSC70 단백질 검출용 PerCP 결합 단클론 항체로 다양한 종에 반응합니다. Western blot, IHC, ICC, IP 등 다중 응용 가능. Chicken HSP70/HSP90 복합체 면역원에서 유래된 BB70 클론. 4°C 보관, 단백질 G 정제, 보존제 무첨가.
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Thermo Fisher Scientific HSP70/HSC70 Monoclonal Antibody (BB70), PerCP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Dog, Chicken, Fruit fly, Fish, Guinea pig, Hamster, Human, Mammal, Mouse, Sheep, Pig, Rabbit, Rat, Xenopus, Yeast |
| Host/Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BB70 |
| Immunogen | Chicken HSP70/HSP90 complex |
| Conjugate | PerCP |
| Excitation/Emission Max | 482/675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.2, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931555 |
Additional Formats
Product Specific Information
1 µg/mL of MA5-45101 was sufficient for detection of HSP70 and HSC70 in 20 µg of heat-shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 72 kDa (HSP) and approximately 73 kDa (HSC).
Target Information
HSPA4 (Heat Shock Protein Family A (Hsp70) Member 4) is a protein-coding gene associated with Chagas Disease and Babesiosis. Related pathways include regulation of activated PAK-2p34 by proteasome-mediated degradation and cellular responses to stimuli. A paralog of this gene is HSPH1.
HSPA8 (Heat Shock Protein Family A (Hsp70) Member 8) is also a protein-coding gene associated with auditory system disease and Borna disease. Related pathways include processing of capped intron-containing pre-mRNA and selective autophagy.
Gene Ontology (GO) annotations include RNA binding and ubiquitin protein ligase binding. A paralog of this gene is HSPA2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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