Thermo Fisher Scientific beta-1 Adrenergic Receptor Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 14 publications 14 publications

Immunohistochemistry (IHC)

Assay-dependent

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

1:100

View 2 publications 2 publications

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Cat, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues H(394) G D R P R A S G C L A R A G(408) of mouse B1AR. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:beta-1 Adrenergic Receptor,uniProtId:P34971-1,ncbiNodeId:10090,antigenRange:395-408,antigenLength:466,antigenImageFileName:PA1-049_beta-1_Adrenergic_Receptor_P34971-1_Rabbit.svg,antigenImageFileNamePDP:PA1-049_beta-1_Adrenergic_Receptor_P34971-1_Rabbit_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2289444

Product Specific Information

PA1-049 detects beta-1 adrenergic receptor (B1AR) from , human, mouse, non-human primate, and rat tissues.

PA1-049 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~50.5 kDa band representing B1AR in mouse kidney membrane preparation. Immunofluorescence staining of B1AR in mouse kidney distal tubule with PA1-049 yields a pattern consistent with plasma membrane staining.

The PA1-049 immunogen is a synthetic peptide corresponding to residues H(394) G D R P R A S G C L A R A G(408) of mouse B1AR. This sequence is completely conserved between mouse and rat B1AR.

Target Information

Adrenergic receptors (ARs) are members of the 7-transmembrane domain G-protein-coupled receptor superfamily that bind the endogenous catecholamines epinephrine and norepinephrine. Pharmacological, structural, and molecular cloning data indicate significant heterogeneity within this receptor family. Nine receptor subtypes have been identified thus far including three alpha-1 AR subtypes (1A/D, 1B, and 1C), three alpha-2 ARs (2A, 2B, and 2C), and three beta AR subtypes (1, 2, and 3). ARs participate in either the onset or maintenance of several disease states including hypertension, cardiac dysfunction (congestive heart failure, ischemia, arrhythmias), diabetes, glaucoma, depression, and impotence. BARs participate in diverse processes including development, behavior, cardiac function, smooth muscle tone, and metabolism. In gene-knockout experiments, the majority of mice that lack the B1AR gene die prenatally and those that do survive until adulthood display abnormal cardiac function. Other studies have shown that the direct regulation of cardiac B1AR density by thyroid hormones occurs at the transcriptional level and is modulated by the catecholamine sensitive-adenylyl cyclase system. Evidence for the effects of cAMP on B1AR regulation has come from a study of members of the cAMP response element (CRE) modulator (CREM) family of transcription factors in transformed cell lines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.