Thermo Fisher Scientific Leptin Polyclonal Antibody
인간, 마우스, 돼지에서 Leptin을 검출하는 Rabbit Polyclonal Antibody. Western blot 및 ICC/IF에 적합하며, 약 16 kDa의 Leptin 단백질을 인식. 합성 펩타이드 면역원 기반, PBS 버퍼에 보관, 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:5,000 | View 7 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | – | View 2 publications |
| Immunocytochemistry (ICC/IF) | 1:20–1:200 | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Pig |
| Published Species | Human, Mouse, Pig, Sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to residues S(91) R N V I Q I S N D L E N L R D(106) of human Leptin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_325787 |
Product Specific Information
- PA1-052 detects leptin from human, mouse, and porcine samples.
- Successfully used in Western blot and ICC/IF applications.
- Detects an ~16 kDa leptin protein from human serum by Western blot.
- Immunogen sequence (S(91)–D(106)) is 94% conserved between human and bovine.
Target Information
Leptin is a 167-amino-acid protein hormone (~16 kDa) that regulates body weight, metabolism, and reproductive function. It is encoded by the obese (ob) gene and primarily secreted by adipocytes, with smaller amounts from stomach epithelium and placenta. Leptin receptors are abundant in the hypothalamus, T lymphocytes, and vascular endothelial cells, mediating effects on feeding behavior, energy expenditure, and thermogenesis.
Leptin also influences hematopoiesis, angiogenesis, wound healing, inflammation, immune responses, and fetal growth. Dysregulation of leptin signaling is associated with obesity, anorexia nervosa, insulin resistance, and hypertension.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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