Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), eFluor 450, eBioscience

Applications and Tested Dilutions

Product Specifications

Additional Available Formats

Unconjugated, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV/Violet/Yellow series, eFluor 506/660, FITC, PE, PE-Cyanine5/7, PE-eFluor 610, PerCP-eFluor 710, Super Bright series, NovaFluor series, Functional Grade, Custom conjugation available.

Product Specific Information

The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by most thymocytes, a subpopulation of mature T cells, and dendritic cells. CD4 binds to MHC class II molecules on antigen-presenting cells and plays a critical role in T cell development and function. In T cells, CD4 associates with protein tyrosine kinase p56lck via its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Flow cytometric analysis of mouse splenocytes (≤0.25 µg/test; 100 µL final volume; cell number empirically determined 10⁵–10⁸ cells/test)
• Fluorophore Information: eFluor® 450 is an alternative to Pacific Blue®, emits at 445 nm, excited at 405 nm (Violet laser)
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD4 antigen is involved in the recognition of MHC class II molecules and acts as a co-receptor for HIV. It is primarily expressed in T helper lymphocytes, but also in monocytes, macrophages, and dendritic cells. Tissue-level expression includes thymus, lymph nodes, tonsils, spleen, and certain regions of the brain and gut. CD4 participates in early T-cell activation via association with the T-cell receptor complex and tyrosine kinase Lck and may mediate neuronal damage in immune-related CNS diseases. Multiple alternatively spliced transcripts have been identified (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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