Thermo Fisher Scientific CD8b Monoclonal Antibody (eBioH35-17.2 (H35-17.2)), eFluor 450, eBioscience

Thermo Fisher Scientific CD8b Monoclonal Antibody (eBioH35-17.2 (H35-17.2)), eFluor 450, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• APC
• APC-eFluor 780
• FITC
• PE
• PE-Cyanine5
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The eBioH35-17.2 monoclonal antibody reacts with the mouse CD8 beta molecule. The CD8 beta chain associates with the CD8 alpha chain to form the CD8 alpha/beta heterodimer expressed on thymocytes and peripheral cytotoxic alpha beta TCR T cells. CD8 binds to MHC class I and plays a role in T cell development and activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Flow cytometric analysis of mouse splenocytes at ≤ 0.25 µg/test
• Recommended Use: Titrate for optimal performance
• Fluorophore Info: eFluor® 450 (alternative to Pacific Blue®)
  • Excitation: 405 nm
  • Emission: 445 nm
  • Laser: Violet Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

The CD8B antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes, mediating efficient immune cell interactions. CD8 acts as a coreceptor with the T-cell receptor to recognize antigens presented by class I MHC molecules. The functional receptor can be a homodimer (alpha-alpha) or heterodimer (alpha-beta). Both chains share homology with immunoglobulin light chains. The CD8B gene encodes the beta chain isoforms, with multiple alternatively spliced variants and a pseudogene on chromosome 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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