Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), eFluor 450, eBioscience

Applications and Tested Dilutions

Product Specifications

Additional Available Formats

Unconjugated, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 561, Alexa Fluor 660, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV series, Brilliant Violet series, eFluor 506/615/660, FITC, Functional Grade, NovaFluor Blue/Red/Violet/Yellow series, PE, PE-Cyanine series, PerCP series, Super Bright series, Custom conjugation available.

Product Specific Information

The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule, a 32–34 kDa cell surface receptor expressed as either a heterodimer (CD8αβ) or homodimer (CD8αα).

• CD8αβ is expressed on most thymocytes and mature αβ TCR T cells.
• CD8αα is expressed on γδ TCR T cells, intestinal intraepithelial lymphocytes (IELs), and dendritic cells.
  CD8 binds to MHC class I and, via association with p56lck, plays a key role in T cell development and activation.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes; optimal use ≤ 0.25 µg/test (100 µL final volume).
Fluorophore: eFluor® 450 (alternative to Pacific Blue®).
Excitation: 405 nm; Emission: 445 nm; Laser: Violet.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD8 (Cluster of Differentiation 8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family.

• Functions as a co-receptor with TCR for MHC class I molecules.
• Expressed on T cells, NK cells, monocytes, and dendritic cells.
• CD8α chain is essential for MHC-I binding.
• Engagement with MHC-I/peptide complexes triggers Lck activation, leading to cytokine production, cytotoxic T lymphocyte (CTL) activation, and immune response regulation.
• Differentiation of naive CD8+ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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