Thermo Fisher Scientific SOX2 Recombinant Rat Monoclonal Antibody (Btjce), Alexa Fluor Plus 488

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

-

Immunocytochemistry (ICC/IF)

1:500

-

Flow Cytometry (Flow)

0.063-0.25 µg/test

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

Btjce

Conjugate

Alexa Fluor™ Plus 488 Alexa Fluor™ Plus 488 Alexa Fluor™ Plus 488

View additional formats

• Unconjugated
• Alexa Fluor Plus 555
• Alexa Fluor Plus 594
• Alexa Fluor Plus 647
• HRP
• Request custom conjugation

Excitation/Emission Max

493/518 nm View spectra

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

proprietary buffer, pH 6.8

Contains

0.008% Bromonitrodioxane, 0.008% Methylisothiazolone

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

RRID

AB_3099298

Product Specific Information

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

Applications Tested: This Btjce antibody has been tested by immunohistochemistry of mouse brain tissue and immunocytochemistry and flow cytometric analysis of NTERA-2 cells. This may be used for immunocytochemistry at 2 µg/mL and for flow cytometry at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 494 nm; Emission: 519 nm; Laser: Blue Laser

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.