Thermo Fisher Scientific SOX2 Recombinant Rat Monoclonal Antibody (Btjce)
SOX2 단백질을 표적하는 재조합 랫드 단클론 항체로, Western blot, IHC, ICC/IF, Flow Cytometry에 사용 가능. 높은 특이성과 일관된 로트 간 재현성 제공. 줄기세포 유지 및 발달 연구에 적합. 단백질 G 정제, PBS 버퍼, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL |
| Immunocytochemistry (ICC/IF) | 1:250–1:1,000 |
| Flow Cytometry (Flow) | 0.002–0.25 µg/test |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host/Isotype | Rat / IgG2a, kappa |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | Btjce |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_3093719 |
Available Conjugate Formats
- Alexa Fluor Plus 488
- Alexa Fluor Plus 555
- Alexa Fluor Plus 594
- Alexa Fluor Plus 647
- HRP
- Custom conjugation available upon request
Product Specific Information
Recombinant rat monoclonal antibodies are produced using in vitro expression systems. These antibodies are generated by cloning specific genes coding for the desired antibodies into expression vectors and expressing them in vitro.
Advantages include improved specificity and lot-to-lot consistency.
It is recommended to titrate the antibody carefully for optimal performance in each assay.
Target Information
SOX2 is an intronless gene encoding a transcription factor belonging to the SRY-related HMG-box (SOX) family. It plays a key role in embryonic development and cell fate determination. The SOX2 protein is essential for stem-cell maintenance in the central nervous system and regulates gene expression in the stomach.
Located within an intron of the SOX2 overlapping transcript (SOX2OT) gene, SOX2 can act as a transcriptional activator through complex formation with other proteins.
Mutations in SOX2 are associated with bilateral anophthalmia, optic nerve hypoplasia, and syndromic microphthalmia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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