Thermo Fisher Scientific LIN28A Recombinant Rabbit Monoclonal Antibody (JA10-63)
LIN28A 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, Flow cytometry, Immunoprecipitation에 적합합니다. 높은 특이성과 재현성을 제공하며, 동물 유래 성분이 없는 포뮬레이션으로 안정적입니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Flow Cytometry (Flow) | 1:50–1:100 |
| Immunoprecipitation (IP) | 1:10–1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JA10-63 |
| Immunogen | Synthetic peptide within Human Lin28 (aa 10–59) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809950 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broad immunoreactivity due to the large rabbit immune repertoire
Target Information
Lin28 is a transcription factor first identified for its role in developmental timing in C. elegans. Its function in development suggests utility in generating stem cells for cell replacement therapies.
Induced pluripotent stem (iPS) cells can be created by expressing Lin28 together with transcription factors POU5F1, Sox2, and NANOG in mouse fibroblasts.
Recent studies show that iPS cells can also be generated using plasmids expressing Lin28, Sox2, POU5F1, and c-Myc, eliminating viral vectors and enhancing safety for regenerative medicine applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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