Thermo Fisher Scientific Alpha Sarcoglycan Recombinant Rabbit Monoclonal Antibody (JA51-81)
Alpha Sarcoglycan을 인식하는 재조합 토끼 단클론 항체로, WB, IHC, IP에 사용 가능. 인간, 마우스, 랫트 반응성. 높은 특이성과 민감도, 로트 간 일관성, 동물 유래 성분 없음. 장기 보관 시 -20°C에서 보관 권장.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1000 |
| Immunoprecipitation (IP) | 1–2 µg/sample |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JA51-81 |
| Immunogen | Synthetic peptide within Human alpha sarcoglycan aa 338–387 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809949 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, and individual clones are screened to select optimal candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
The sarcoglycan transmembrane proteins are part of the dystrophin complex. Sarcoglycans form a membrane-associated complex in skeletal, cardiac, and smooth muscle fibers.
Four sarcoglycan subunits—alpha, beta, gamma, and delta—form the skeletal muscle cell surface complex. Mutations in any of these genes lead to loss or reduction of the entire sarcoglycan complex, causing autosomal recessive muscular dystrophy (sarcoglycanopathy).
In smooth muscle, beta- and delta-sarcoglycans associate with epsilon-sarcoglycan, homologous to alpha-sarcoglycan. Delta-sarcoglycan deficiency is linked to Syrian hamster BIO.14 cardiomyopathy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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