Thermo Fisher Scientific ITGA4 Recombinant Rabbit Monoclonal Antibody (JA09-36)
ITGA4 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, Flow cytometry에 적합. 높은 특이성과 재현성을 제공하며, 동물 유래 성분이 없는 포뮬레이션. 장기 보관 시 -20°C에서 안정적 유지.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:10–1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JA09-36 |
| Immunogen | Synthetic peptide within Human Integrin alpha 4 (aa 983–1032), target family: CD49d (Integrin alpha 4), UniProt ID: P13612-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809954 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Specific antibody DNA sequences from immunoreactive rabbits are cloned and expressed, followed by screening for optimal clones.
Key advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
Integrin alpha 4 (CD49d, ITGA4) is part of the integrin alpha chain family. Integrins are heterodimeric membrane proteins composed of alpha and beta chains.
The alpha4 chain associates with either beta1 or beta7 chains. Ligand-binding sites for integrin alpha4β1 and alpha4β7 are located on the alpha4 chain, binding ligands such as MadCAM, VCAM, and fibronectin.
MadCAM is the principal ligand for integrin alpha4β7. Additionally, the HIV-1 envelope can mimic MadCAM by binding to and signaling through integrin alpha4β7, a gut mucosal homing receptor for peripheral T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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