Thermo Fisher Scientific SFRP1 Recombinant Rabbit Monoclonal Antibody (JA11-68)
SFRP1 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC, IP 등 다양한 응용에 적합. 높은 특이성과 재현성을 제공하며, HEK293 세포에서 발현된 동물 유래 성분 무첨가 포뮬레이션. 단기 4°C, 장기 -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JA11-68 |
| Immunogen | Synthetic peptide within Human SFRP1 aa 31–80 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809952 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences are cloned from immunoreactive rabbits, and individual clones are screened to select optimal candidates for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
Secreted frizzled-related protein 1 (SFRP1) is part of the SFRP family, containing a cysteine-rich domain similar to the Wnt-binding site of Frizzled proteins.
SFRPs act as soluble modulators of Wnt signaling. SFRP1 and SFRP5 may influence photoreceptor cell polarity in the retina.
SFRP1 is expressed in various human tissues, with the highest levels in the heart.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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