Thermo Fisher Scientific H3K27ac Polyclonal Antibody
H3K27ac 폴리클로날 항체로, Western blot, IHC, ICC, IP, ChIP 등 다양한 응용에 사용 가능. Human, Mouse, Rat 반응성. Rabbit IgG 기반, 항원 친화 크로마토그래피로 정제. 액상 형태로 제공되며, 4°C 또는 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
| Immunoprecipitation (IP) | 1:100–1:5,000 | - |
| ChIP assay (ChIP) | Assay-dependent | 1 publication |
| Dot blot (DB) | Assay-dependent | - |
| Immunomicroscopy (IM) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H3K27ac (acetyl Lys27). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.53 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792664 |
Product Specific Information
- Keep as concentrated solution.
- Positive Control: HeLa.
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the structure of the nucleosome.
Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.
Post-translationally, histones are modified in various ways to either directly change chromatin structure or allow for binding of specific transcription factors.
The N-terminal tail of histone H3 can undergo multiple post-translational modifications such as methylation, acetylation, and phosphorylation, influencing diverse cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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