Thermo Fisher Scientific SLUG Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:10,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human SLUG.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.18 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2792662

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Mouse (99%), Rat (98%), Zebrafish (85%), Xenopus laevis (95%), Dog (100%), Pig (97%), Sheep (98%), Rhesus Monkey (100%), Bovine (99%).

Positive Control: human Slug-transfected 293T cells, U87-MG, SK-N-SH, IMR32, SK-N-AS, NT2D1.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Slug, a member of the Snail family of C2H2-type zinc finger transcription factors, was initially identified to be involved in epithelial-mesenchymal transitions as well as the formation of the neural tube during vertebrate embryogenesis. Like Snail, Slug transcription can be induced by growth factors such as FGF, BMP, and TGF-beta. Once expressed, Slug will bind E-box regions of promoters and repress transcription of genes such as E-cadherin and Claudin-1. More recently, its expression in breast, esophogeal, and colorectal carcinomas has been correlated with poor prognosis for survival. Furthermore, Slug can protect hemopoietic progenitor cells from radiation-induced apoptosis by repressing the p53-mediated transcription of Puma, a BH3-only antagonist of the anti-apoptotic members of the Bcl-2 family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.