Thermo Fisher Scientific H3K4me3 Polyclonal Antibody
H3K4me3 폴리클로날 항체로, 인간·마우스·랫·제브라피쉬 시료에 반응합니다. Western blot, IHC, ICC, ChIP 등 다양한 응용에 사용 가능하며, 항원 친화 크로마토그래피로 정제되었습니다. PBS 버퍼에 보관되며 연구용으로 적합합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | |
| Immunohistochemistry - Free Floating (IHC (Free)) | 1:100–1:500 | |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | |
| ChIP assay (ChIP) | Assay-dependent | |
| Dot blot (DB) | Assay-dependent | |
| Immunomicroscopy (IM) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat, Zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H3K4me3 (trimethyl Lys4). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.12 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792665 |
Product Specific Information
- Keep as concentrated solution.
- Positive Control: HeLa nuclear, whole zebrafish.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Post-translationally, histones are modified in various ways to alter chromatin structure or facilitate transcription factor binding. The N-terminal tail of histone H3 can undergo modifications such as methylation, acetylation, and phosphorylation, influencing diverse cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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