Thermo Fisher Scientific H3K18ac Polyclonal Antibody, ChIP-Verified
H3K18ac에 특이적인 Rabbit Polyclonal 항체로, ChIP 검증 완료. WB, IHC, ICC, IP 등 다양한 응용에 사용 가능. Human, Mouse, Rat, Yeast 반응성. 항원 친화 크로마토그래피로 정제된 고품질 항체. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | 1:100–1:500 |
| ChIP assay (ChIP) | Assay-dependent |
| Dot blot (DB) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat, Yeast |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H3K18ac (acetyl Lys18). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.26 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792663 |
Product Specific Information
- Keep as concentrated solution.
- Positive Control: HeLa.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Master Lot linking information (PDF)
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Together with other core histones, it binds to DNA to form nucleosomes, the fundamental units of chromatin structure. Histones are crucial for transcription regulation, DNA repair, replication, and chromosomal stability.
The N-terminal tail of histone H3 can undergo various post-translational modifications—such as methylation, acetylation, and phosphorylation—that influence chromatin structure and gene expression.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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