Thermo Fisher Scientific E2F2 Recombinant Rabbit Monoclonal Antibody (SN201-04)
E2F2 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot 및 유세포분석에 적합합니다. 높은 특이성과 일관된 로트 간 품질을 제공하며, HEK293 세포에서 발현된 IgG 형식의 항체입니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SN201-04 |
| Immunogen | Recombinant protein within Human E2F2 aa 283–415 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809615 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Specific antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates for production.
Advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
E2F2 is a member of the E2F family of transcription factors, which play a crucial role in cell cycle control and tumor suppressor protein function.
E2F proteins contain conserved domains including:
- DNA-binding domain
- Dimerization domain for DP protein interaction
- Transactivation domain rich in acidic amino acids
- Tumor suppressor protein association domain
E2F2, along with E2F1 and E2F3, possesses an additional cyclin-binding domain. It binds specifically to the retinoblastoma protein (pRB) in a cell cycle–dependent manner and shares approximately 46% amino acid identity with E2F1.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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