Thermo Fisher Scientific Cytokeratin 4 Recombinant Rabbit Monoclonal Antibody (SN2001)
Cytokeratin 4 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC에 적합. 높은 특이성과 재현성을 제공하며, HEK293 발현 시스템에서 생산됨. 단백질 A 정제, 1 mg/mL 농도, 동물 유래 성분 없음.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SN2001 |
| Immunogen | Synthetic peptide within Human Cytokeratin 4 (aa 1–50) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809618 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and expressed, and the best clones are selected for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
Cytokeratins are a subfamily of intermediate filament proteins with high biochemical diversity, represented by at least 20 different polypeptides in human epithelial tissues.
They range in molecular weight from approximately 40 kDa to 68 kDa and have isoelectric points between pH 4.9–7.8.
Cytokeratin expression patterns are characteristic of epithelial type and differentiation stage, making them valuable for distinguishing epithelial malignancies.
Cytokeratin antibodies are useful in:
- Differential diagnosis of tumors via immunohistochemistry
- Cytopathology and flow cytometry assays
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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