Thermo Fisher Scientific NPHP1 Polyclonal Antibody
Thermo Fisher Scientific의 NPHP1 Polyclonal Antibody는 인간, 마우스, 랫트 시료에 반응하는 rabbit IgG 항체로, Western blot에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 1 mg/mL 농도의 액상 제품입니다. 4°C 단기 보관, -20°C 장기 보관 권장.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to residues in human Nephrocystin-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2553949 |
Product Specific Information
- Detects endogenous protein at a molecular weight of 83 kDa.
- Purity is >95% by SDS-PAGE.
Target Information
Juvenile nephronophthisis type 1 is caused by mutations of NPHP1, the gene encoding for nephrocystin.
Nephrocystin interacts with p130Cas (BCAR1), proline-rich tyrosine kinase-2 (PTK2B or Pyk2), and tensin in embryonic kidney and testis, suggesting participation in a common signaling pathway.
Nephrocystin and p130Cas interact in mammalian cells and localize at or near sites of cell-cell contact in polarized Madin-Darby canine kidney epithelial cells.
Expression of nephrocystin results in phosphorylation of Pyk2 on Tyrosine 402 and activation of downstream MAP kinases (ERK1, ERK2).
Nephrocystin contains a conserved src-homology 3 (SH3) domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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