Thermo Fisher Scientific Siglec-G Monoclonal Antibody (SH2.1), PerCP-eFluor 710, eBioscience
마우스 Siglec-G 단백질을 인식하는 SH2.1 단클론 항체로, PerCP-eFluor 710 형광 표지. Flow cytometry에 최적화되어 있으며 B세포 특이적 발현 연구에 적합. 488 nm 레이저로 여기되어 710 nm에서 방출. 4°C 암소 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | ≤ 0.125 µg/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor™ 710, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SH2.1 |
| Conjugate | PerCP-eFluor™ 710 |
| Excitation/Emission Max | 482/708 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573777 |
Additional Formats
Product Specific Information
Description:
The SH2.1 monoclonal antibody reacts with mouse Siglec-G (sialic acid-binding immunoglobulin-like lectin-G). Siglec-G is an inhibitory receptor expressed specifically in B cells, with B1 B cells showing the highest expression levels. Overexpression of Siglec-G inhibits B cell receptor-mediated calcium signaling. Siglec-G-deficient mice display expansion of the B1a cell population and increased natural IgM antibody titers. These cells exhibit decreased spontaneous apoptosis and prolonged lifespan, potentially due to elevated NFATc1 expression. Siglec-G-dependent negative regulation explains the muted signaling response of B1 cells.
Applications Reported:
This SH2.1 antibody has been reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometry using mouse splenocytes. Recommended use is ≤ 0.125 µg per test (in 100 µL final volume). Optimal titration should be empirically determined.
Fluorophore Information:
PerCP-eFluor® 710 emits at 710 nm and is excited with the 488 nm blue laser. It can substitute for PerCP-Cyanine5.5. Recommended filter: 710/50 bandpass (695/40 acceptable alternative). Ensure instrument compatibility.
Fixation:
Samples may be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark. Clone-specific performance should be empirically verified.
Optical Properties:
- Excitation: 488 nm
- Emission: 710 nm
- Laser: Blue Laser
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
Siglec-G is an inhibitory receptor expressed in B cells, particularly B1 cells. Overexpression inhibits B cell receptor-mediated calcium signaling. Siglec-G-deficient mice show B1a cell expansion, elevated natural IgM titers, reduced apoptosis, and prolonged lifespan due to increased NFATc1 expression. This receptor mediates negative regulation in B1 cells, contributing to their muted signaling response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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