Thermo Fisher Scientific TCR V alpha 2 Monoclonal Antibody (B20.1), PerCP-eFluor 710, eBioscience

Thermo Fisher Scientific TCR V alpha 2 Monoclonal Antibody (B20.1), PerCP-eFluor 710, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.125 µg/test
Publications: View 4 publications

Product Specifications

Additional Formats

• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• NovaFluor Blue 610-70S
• NovaFluor Red 700
• NovaFluor Yellow 570
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Request custom conjugation

Product Specific Information

The B20.1 monoclonal antibody reacts with mouse T-cell receptor (TCR) V alpha 2 chains. It recognizes the majority of the TCR V alpha 2 subfamily in mice carrying a, b, and c haplotypes and also reacts with TCR V delta 8 products due to high homology.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤0.125 µg/test (100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test. Antibody titration recommended for optimal performance.

PerCP-eFluor™ 710 can replace PE-Cy5, PE-Cy5.5, or PerCP-Cy5.5.

• Excitation: 488 nm (Blue Laser)
• Emission: 710 nm
• Recommended Filters: 685 LP dichroic mirror and 710/40 band pass filter (or 695/40 alternative)

PerCP-eFluor™ 710 conjugated antibodies are stable after exposure to freshly prepared 2% formaldehyde overnight at 4°C.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

T cell receptors (TCR) discriminate foreign from self-peptides presented by MHC class II molecules, crucial for adaptive immunity. Recognition of self-peptides is linked to autoimmune diseases and tumors. Superantigen engagement of TCRs can cause toxic shock syndrome. Autoantibodies to TCR V beta segments are found in RA and SLE patients and may modulate immune responses.
Most human T cells express TCR alpha-beta and CD4 or CD8 (SP). A small fraction are double negative (DN), which are increased in certain autoimmune or immunodeficiency disorders. Gamma-delta T cells, mainly in epithelium, show less diversity and unique antigen recognition, with subsets exhibiting antitumor and immunoregulatory functions.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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