Thermo Fisher Scientific Phospho-GSK3 alpha/beta (Tyr279, Tyr216) Recombinant Superclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1–2 µg/mL

Immunocytochemistry (ICC/IF)

• Tested Dilution: 2 µg/mL

Product Specifications

Product Specific Information

This antibody is predicted to react with monkey, rabbit, and horse.

Recombinant rabbit Superclonal™ antibodies are composed of multiple different recombinant monoclonal antibodies, combining the sensitivity of polyclonal antibodies with the specificity and consistency of monoclonal antibodies.
Each batch is produced with a defined mixture of light and heavy chains, ensuring reproducibility and eliminating the variability of traditional polyclonal production.

Note: Formerly known as “Recombinant Polyclonal Antibody”, this product is now branded as “Recombinant Superclonal™ Antibody”. The physical product and performance remain unchanged.

Target Information

Glycogen Synthase Kinase-3 (GSK3) is a protein kinase originally identified as a regulator of glycogen synthase, a key enzyme in glycogen metabolism.
It regulates various cellular processes including:

• Protein synthesis
• Cell proliferation and differentiation
• Microtubule assembly/disassembly
• Apoptosis

GSK3 phosphorylates substrates only when a phosphoserine or phosphotyrosine residue is present four residues downstream of the target site.
There are two isoforms, GSK3 alpha and GSK3 beta, regulated via phosphorylation:

• Inactivation occurs by phosphorylation at Ser9 (Ser21 in GSK3α) by PKB.
• Activation occurs via phosphorylation at Tyr216 (Tyr279 in GSK3α).

Activated GSK3β phosphorylates multiple cellular proteins including p53, c-Myc, c-Jun, HSF-1, and cyclin D1, and is implicated in tau phosphorylation relevant to Alzheimer’s disease progression.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.