Thermo Fisher Scientific IPO-38 Monoclonal Antibody (IPO-38)
인간 반응성 마우스 IgM 단클론 항체로, IPO-38 항원을 인식합니다. WB, IHC, ELISA, IP 등 다양한 응용에 적합합니다. 세포 증식 및 종양 진행 모니터링에 유용한 연구용 시약입니다. -20°C에서 안정적으로 보관 가능합니다.
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Thermo Fisher Scientific IPO-38 Monoclonal Antibody (IPO-38)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 – 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 10 µg/mL |
| ELISA | 1:10,000 – 1:50,000 |
| Immunoprecipitation (IP) | 1:500 – 1:2,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgM, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | IPO-38 |
| Immunogen | A BALB/c mouse was immunized with spleen cells of a human patient with hairy cell leukemia. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.5M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Reacts with a 14–16 kDa protein as demonstrated by western blot analysis on the Raji cell line. This antigen was detected after 12h of PHA-induced activation in the early G1 phase but absent in non-stimulated lymphocytes.
Cross-reactivity is likely to occur with mouse and rat. Reactivity with other sources has not been determined.
Target Information
IPO-38 antigen is present in the nuclei of proliferating cells such as Hodgkin’s disease and non-Hodgkin’s lymphomas, different forms of leukemias, breast and colorectal carcinomas, and PHA-stimulated lymphocytes.
It is not expressed in the cells of non-stimulated lymphocytes and granulocytes. IPO-38 can be a useful marker of cell proliferation during monitoring of tumor progression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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