Thermo Fisher Scientific IPO-38 Monoclonal Antibody (IPO-38)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500- 1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL

-

ELISA (ELISA)

1:10,000-1:50,000

-

Immunoprecipitation (IP)

1:500- 1:2,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgM, kappa

Class

Monoclonal

Type

Antibody

Clone

IPO-38

Immunogen

A BALB/c mouse was immunized with spleen cells of a human patient with hairy cell leukemia.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.5M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Reacts with a 14 -16 kDa protein as demonstrated by western blot analysis on the Raji cell line. This antigen was detected after 12h of PHA-induced activation in the early G1 phase but absent in non-stimulated lymphocytes. Cross reactivity is likely to occur with mouse and rat. Reactivity with other sources has not been determined.

Target Information

IPO-38 antigen is present in the nuclei of proliferating cells such as Hodgkins disease and non-Hodgkins lymphomas, different forms of leukemias, breast and colorectal carcinomas, and PHA-stimulated lymphocytes. It is not expressed in the cells of non-stimulated lymphocytes and granulocytes. IPO-38 can be a useful marker of cell proliferation during monitoring of tumor progression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.