Thermo Fisher Scientific Bcl-2 Monoclonal Antibody (Bcl-2/100), eBioscience
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카탈로그 번호 | CAS 번호 | 설명 | 상태 | 단위 | 판매가 | 할인가 | 가격(VAT포함) | 수량 / 장바구니 / 찜 |
14-1028-37 | - | Thermo Fisher Scientific 14-1028-37 Bcl-2 Monoclonal Antibody (Bcl-2/100), eBioscience 2 mg pk | 재고문의 | pk | 3,033,000원 | - | 3,336,300원 | |
14-1028-82 | - | Thermo Fisher Scientific 14-1028-82 Bcl-2 Monoclonal Antibody (Bcl-2/100), eBioscience 100 ug pk | 재고문의 | pk | 348,000원 | - | 382,800원 |
다른 상품 둘러보기
Applications
Tested Dilution
Publications
Western Blot (WB)
2 µg/mL
View 3 publications 3 publications
Immunohistochemistry (IHC)
Assay-Dependent
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse / IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
Bcl-2/100
Conjugate
Unconjugated Unconjugated Unconjugated
View additional formats
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2864958
Product Specific Information
Description: This bcl-2/100 monoclonal antibody reacts to human Bcl-2 (B-cell Lymphoma 2), the founding member of a family of pro-apoptotic proteins which includes Bcl-XL, Bcl-w, and Mcl-1. Bcl-2 plays a role in protecting against apoptosis-inducing insults such as dexamethasone and staurosporine, as well as irradiation and cytokine withdrawal. The mechanism is thought to include regulation of mitochondrial membrane permeability, resulting in sequestration of caspase proforms, or by preventing the release of apoptosis-mediating mitochondrial factors, such as cytochrome c and AIF (apoptosis-inducing factor), into the cytoplasm. Bcl-2 is localized to the cytoplasmic side of the mitochondrial membrane, the nuclear envelope, and the endoplasmic reticulum. Interestingly Bcl-2 does not play a role in preventing cell death of autoreactive T cells in the thymus or Fas-mediated apoptosis. Two isoforms, termed alpha and beta of Bcl-2 have been identified and are a result of alternative splicing at the C-terminus. This antibody recognizes both forms.
Applications Reported: This Bcl-2/100 antibody has been reported for use in immunoblotting (WB).
Applications Tested: This Bcl-2/100 antibody has been tested by immunoblot analysis of the Jurkat cell line. This can be used at 1-5 µg/mL for detection of reduced and denatured protein.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. The BCL-2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with BCL-2 resulting in the enhancement of the anti-apoptotic effect of BCL-2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Two transcript variants of BCL-2, produced by alternate splicing, differ in their C-terminal ends. The overexpression of BCL-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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