Thermo Fisher Scientific PLK1 Antibody Cocktail

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 36 publications 36 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

-

Immunocytochemistry (ICC/IF)

1-5 µg/mL

View 6 publications 6 publications

ELISA (ELISA)

0.1-1.0 µg/mL

-

Immunoprecipitation (IP)

5 µg

View 5 publications 5 publications

Miscellaneous PubMed (Misc)

-

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat, Xenopus

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa/IgG2b, kappa

Class

Cocktail

Type

Antibody

Clone

PL6/PL2

Immunogen

A His-tagged Plk fusion protein (C-term Plk1) containing amino acids 402-603 of the human Plk1 protein( if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PLK1,uniProtId:P53350-1,ncbiNodeId:9606,antigenRange:402-603,antigenLength:603,antigenImageFileName:33-1700_PLK1_P53350-1_House_mouse.svg,antigenImageFileNamePDP:33-1700_PLK1_P53350-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533104

Product Specific Information

33-1700 contains a cocktail of monoclonal mouse PLK1 containing clone PL6, isotype IgG1, kappa and clone PY20, isotype IgG2b, kappa.

Target Information

PLK1 is a serine/threonine kinase that is thought to function in cell mitosis and regarded as a marker of cell proliferation. It has been found to play a critical role in carcinogenesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14, TOPORS, p73/TP73, TPT1 and WEE1. Plays a key role in centrosome functions and the assembly of bipolar spindles by phosphorylating KIZ, NEDD1 and NINL.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.