Thermo Fisher Scientific Occludin Monoclonal Antibody (OC-3F10), HRP
HRP 결합형 Occludin 단일클론 항체로 Western blot, ELISA, ICC/IF에 사용 가능. 인간, 마우스, 랫, 개 반응성 보유. 고순도 Protein A 정제 및 안정적 PBS/BSA/glycerol 저장 buffer 구성. 연구용으로 세포 간 밀착단백질 Occludin 검출에 적합.
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Thermo Fisher Scientific Occludin Monoclonal Antibody (OC-3F10), HRP
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2 µg/mL | View 7 publications |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| ELISA | 0.1–1.0 µg/mL | View 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Published Species | Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OC-3F10 |
| Immunogen | GST fusion protein consisting of the C-terminal region (~150aa) of human occludin |
| Conjugate | HRP |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 4 mg/mL BSA, 40% glycerol |
| Contains | 0.19% Kathon™ CG |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533103 |
Additional Formats
Target Information
Occludin is a 65 kDa protein that can exist in various phosphorylated forms (up to ~82 kDa). It plays a key role in regulating tight junction localization and function. Polyunsaturated fatty acids up-regulate occludin expression, increasing transendothelial resistance and reducing permeability to large molecules. Expression levels vary by tissue—high in brain tissue at cell-cell junctions and lower in non-neural tissues. Structural features of occludin are conserved across species. Under-expression of occludin and other tight junction proteins contributes to increased permeability in tumor endothelial junctions, leading to brain tumor edema.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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