Thermo Fisher Scientific CD326 (EpCAM) Monoclonal Antibody (323/A3), Alexa Fluor 488
EpCAM(CD326)을 인식하는 마우스 단클론 항체로, Alexa Fluor 488 형광으로 표지되어 있습니다. Flow Cytometry, ICC/IF, Western blot 등 다양한 응용에 사용 가능하며, 인간 시료에 반응합니다. EpCAM 과발현 암세포 연구에 적합합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | 4 µL / 1×10⁶ cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 323/A3 |
| Immunogen | Human breast cancer MCF-7 cells |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Purification | Size-exclusion chromatography |
| Storage Buffer | PBS with 0.2% BSA |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898625 |
Additional Formats
- Unconjugated (MA1-10196)
- Alexa Fluor 647 (MA5-38714)
- APC (MA5-38715)
- FITC (MA5-38716)
- PE (MA5-38717)
- PE-Cyanine7 (MA5-38718)
- PerCP-Cyanine5.5 (MA5-38719)
- Custom conjugation available
Product Specific Information
This antibody recognizes an extracellular epitope of CD326 / EpCAM, a marker of epithelial lineages that is over-expressed in many carcinomas.
Target Information
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues. It functions as a calcium-independent cell adhesion molecule and influences cell cycle, proliferation, and metabolism by inducing genes such as c-myc, cyclin A, and E.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth. Overexpression of Ep-CAM is linked to enhanced epithelial proliferation and is a marker for epithelial-derived tumors. It is expressed on the baso-lateral surface of most simple epithelia and many carcinomas, and helps distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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