Thermo Fisher Scientific Cofilin Monoclonal Antibody (GT217)
Cofilin 단백질을 인식하는 Mouse monoclonal antibody로 Western blot, IHC, ICC, IP 등 다양한 응용에 사용 가능. Human, Mouse, Rat 반응성. 단백질 A로 정제된 액상 형태로 제공되며, 4°C 또는 -20°C 보관 권장. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | - |
| Immunoprecipitation (IP) | 1:100–1:500 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT217 |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cofilin 1 (exact sequence proprietary) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2735151 |
Product Specific Information
- Positive Control: 293T, A431, HeLa, HepG2, PC-12, Rat-2, Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12
- Predicted Reactivity: Pig (99%), Sheep (99%), Rhesus Monkey (99%), Bovine (99%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Cofilin binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. It regulates actin cytoskeleton dynamics and is essential for normal mitosis and cytokinesis. Cofilin plays a role in cell morphology and cytoskeletal organization, and is required for the regulation of atypical chemokine receptor ACKR2 trafficking, enhancing chemokine uptake and degradation. It is also necessary for neural tube morphogenesis and neural crest cell migration.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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