Thermo Fisher Scientific Cofilin Monoclonal Antibody (GT217)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Immunoprecipitation (IP)

1:100-1:500

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

GT217

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cofilin 1. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2735151

Product Specific Information

Positive Control: 293T, A431, HeLa, HepG2, PC-12, Rat-2, Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12

Predicted Reactivity: Pig (99%), Sheep (99%), Rhesus Monkey (99%), Bovine (99%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Cofilin binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. Required for neural tube morphogenesis and neural crest cell migration.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.