Thermo Fisher Scientific ATP6V1A Monoclonal Antibody (GT3846)
ATP6V1A 단백질을 표적하는 Mouse IgG2a 단일클론 항체로, Human, Mouse, Rat 반응성. WB, IHC, Flow Cytometry에 적합하며, Protein A로 정제된 액상 형태. 세포 내 산성화 관련 연구에 사용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Flow Cytometry (Flow) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT3846 |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ATP6V1A (aa 85–339) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.44 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2735123 |
Product Specific Information
- Positive Control: 293T, HepG2
- Predicted Reactivity: Xenopus laevis (90%), Pig (96%), Chicken (83%), Rhesus Monkey (99%), Bovine (98%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ATP6V1A encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles.
V-ATPase dependent organelle acidification is necessary for intracellular processes such as:
- Protein sorting
- Zymogen activation
- Receptor-mediated endocytosis
- Synaptic vesicle proton gradient generation
V-ATPase is composed of:
- V1 domain: Three A and three B subunits, two G subunits plus C, D, E, F, and H subunits (contains ATP catalytic site)
- V0 domain: Five different subunits (a, c, c′, c″, and d)
Additional isoforms of many V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants.
This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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