Thermo Fisher Scientific TET1 Monoclonal Antibody (OTI8C3), TrueMAB
인간 TET1 단백질(aa 1-305)을 항원으로 제작된 Mouse IgG1 단클론 항체. Western blot에 1:2,000 희석으로 사용 가능. 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도로 사용. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI8C3 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–305 of human TET1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
TET1 is a dioxygenase that specifically binds methylcytosine (5mC), a minor base in mammalian DNA essential for retrotransposon silencing and development.
It catalyzes the conversion of 5mC to 5-hydroxymethylcytosine (hmC), which may influence chromatin structure, recruit specific factors, or serve as an intermediate in cytosine demethylation.
5hmC is enriched in embryonic stem cells and brain tissue, particularly Purkinje neurons, and may play roles in fetal development of the heart, lung, and brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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