Thermo Fisher Scientific HRI Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 337-586 of human EIF2AK1 (NP_0552282) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HRI,uniProtId:Q9BQI3-1,ncbiNodeId:9606,antigenRange:337-586,antigenLength:630,antigenImageFileName:PA5-89632_HRI_Q9BQI3-1_Rabbit.svg,antigenImageFileNamePDP:PA5-89632_HRI_Q9BQI3-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.93 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2805700

Product Specific Information

Positive Samples: A-431, SH-SY5Y; Cellular Location: Cytoplasm

Immunogen sequence: SIVEQQLPLR RNSHLEESFT STEESSEENV NFLGQTEAQY HLMLHIQMQL CELSLWDWIV ERNKRGREYV DESACPYVMA NVATKIFQEL VEGVFYIHNM GIVHRDLKPR NIFLHGPDQQ VKIGDFGLAC TDILQKNTDW TNRNGKRTPT HTSRVGTCLY ASPEQLEGSE YDAKSDMYSL GVVLLELFQP FGTEMERAEV LTGLRTGQLP ESLRKRCPVQ AKYIQHLTRR NSSQRPSAIQ LLQSELFQNS

Target Information

EIF2AK1 (HRI) is one of 4 kinases that specifically phosphorylate Ser51 of translation initiation factor eIF2-alpha in response to various environmental stresses, leading to a decrease in protein sythesis. In the case of EIF2AK1, signaling is initiated by low levels of heme. The HRI gene is localized to 7p22 where its 3end slightly overlaps the 3 end of the gene JTV1. The two genes are transcribed from opposite strands and kinase activity is induced by low levels of heme and inhibited by the presence of heme.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.