
Thermo Fisher Scientific Fibronectin (Total) Monoclonal Antibody (C6F10)
Fibronectin 단백질을 검출하기 위한 Thermo Fisher Scientific의 C6F10 단클론 항체로, 다양한 종에 반응하며 WB, IHC, ICC, IP 등에 사용 가능. 단일형 IgG1 항체로, Protein A/G 정제 및 PBS 용액 형태로 제공. 연구용으로 적합.
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Thermo Fisher Scientific Fibronectin (Total) Monoclonal Antibody (C6F10)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Immunoprecipitation (IP) | 1–2 µg per 100–500 µg of protein |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Amphibian, Avian, Bovine, Horse, Human, Non-human primate, Pig |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | C6F10 |
| Immunogen | Human plasma fibronectin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Fibronectin is a disulfide-bonded dimer with a molecular weight range of 230–250 kDa. In the extracellular matrix of connective tissues and vessels, fibronectin exists as an insoluble, cross-linked polymer. It plays a key role in embryonic development, tissue remodeling, wound healing, blood coagulation, host defense, and metastasis.
Fibronectin is abundant in plasma as a soluble dimer and in tissues as dimeric or multimeric forms. Its expression increases in non-small cell lung carcinoma, enhancing tumorigenicity and resistance to apoptosis. The gene encoding fibronectin undergoes alternative splicing, potentially producing up to 20 transcript variants.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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