Thermo Fisher Scientific Phospho-PAK1/PAK2 (Ser199, Ser204, Ser192, Ser197) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human PAK1(Accession Q13153), corresponding to amino acid residues around phosphorylated Ser204. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PAK1/PAK2,uniProtId:Q13153-1,ncbiNodeId:9606,antigenRange:204,antigenLength:545,antigenImageFileName:PA5-104628_PAK1PAK2_Q13153-1_Rabbit.svg,antigenImageFileNamePDP:PA5-104628_PAK1PAK2_Q13153-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2816103

Product Specific Information

Antibody detectsAntibody detects endogenous levels of PAK1 when phosphorylated at Ser199/204 or PAK2 when phosphorylated at Ser192/197.

Target Information

Three isoforms of serine/threonine kinases, designated alphaPAK p68, betaPAK p65 and gammaPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The alpha, beta and gammaPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on gammaPAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with gammaPAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.