Thermo Fisher Scientific Phospho-p53 (Thr18) Polyclonal Antibody
인간, 생쥐, 랫트의 인산화된 p53(Thr18)을 검출하는 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF에 적합하며, 고순도 액상 형태로 PBS/glycerol buffer에 보존. 세포 스트레스 반응 및 암 연구용으로 최적화된 연구용 시약.
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Thermo Fisher Scientific Phospho-p53 (Thr18) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human TP53 (Accession P04637), corresponding to amino acid residues around phosphorylated Thr18 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816101 |
Product Specific Information
This antibody detects endogenous levels of p53 only when phosphorylated at Thr18.
Target Information
The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or nutrient deprivation.
p53 activation leads to transcription of genes such as p21waf1, regulating G1 or G2/M arrest. Structurally, p53 comprises:
- N-terminal transactivation domain
- Central DNA-binding domain
- Oligomerisation domain
- C-terminal regulatory domain
Phosphorylation at Ser15 is crucial for activation and stabilization, while phosphorylation at Ser392 is associated with tumor formation. p53 also influences aging, oxidative stress, and memory processes.
In normal cells, p53 is expressed at low levels, but is elevated in transformed cell lines, contributing to malignancy. Mutations in TP53 disrupt DNA binding and tumor suppressor activity, and occur in both somatic and germline forms (e.g., Li-Fraumeni syndrome).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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