Thermo Fisher Scientific PLCG1 Monoclonal Antibody (M156)
PLCG1 단백질을 검출하기 위한 Thermo Fisher Scientific의 Mouse Monoclonal Antibody (Clone M156). Western blot, ICC/IF, IP에 적합하며 Human, Mouse, Rat 시료 반응성. Protein A 정제, PBS/BSA/glycerol buffer, -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M156 |
| Immunogen | Clone (M156) was generated from a synthetic peptide corresponding to amino acids in the N-terminal region of human PLCγ1. This sequence is highly conserved in rat and mouse PLCγ1, and has low homology to PLCγ2. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | ~0.12 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2942659 |
Product Specific Information
The antibody detects a 150 kDa protein in human A431, Hct116, and Jurkat cells, as well as in mouse brain.
Target Information
The mammalian Phospholipase Cγ (PLCγ) family includes two closely related proteins, PLCγ1 and PLCγ2. These isozymes share core structural domains and contain unique regions with an additional PH domain, two SH2 domains, and one SH3 domain. In response to extracellular stimuli such as hormones and growth factors, receptor tyrosine kinases (RTKs) phosphorylate and activate PLCγ. Activated PLCγ catalyzes hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) to produce the second messenger molecules inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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