Thermo Fisher Scientific PLCG1 Monoclonal Antibody (M156)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1000

-

Immunocytochemistry (ICC/IF)

1:100

-

Immunoprecipitation (IP)

1:100

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

M156

Immunogen

Clone (M156) was generated from a synthetic peptide corresponding to amino acids in the N-terminal region of human PLCgamma1. This sequence is highly conserved in rat and mouse PLCgamma1, and has low homology to PLCgamma2.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

~0.12 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2942659

Product Specific Information

The antibody detects a 150 kDa* protein in human A431, Hct116, and Jurkat cells, as well as in mouse brain.

Target Information

The mammalian Phospholipase Cg (PLCg) family has two closely related proteins, PLCg1 and PLCg2. The PLCg isozymes have the core structure domains and a unique array of domains containing an additional PH domain, two SH2 domains and one SH3 domain. In response to extracellular stimuli, such as hormones and growth factors, receptor tyrosine kinases (RTKs) phosphorylate and activate PLCg. Activated PLCg catalyzes hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) to produce the metabolic second messenger molecules inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.