Thermo Fisher Scientific TCR beta Monoclonal Antibody (H57-597), FITC, eBioscience

Thermo Fisher Scientific TCR beta Monoclonal Antibody (H57-597), FITC, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 496
• Brilliant UV 661
• Brilliant UV 737
• Brilliant Violet 421
• eFluor 450
• eFluor 506
• Functional Grade
• NovaFluor Blue 510
• NovaFluor Blue 530
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 725
• NovaFluor Blue 800
• NovaFluor UV 765
• NovaFluor Violet 690
• NovaFluor Violet 800
• NovaFluor Yellow 810
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Custom conjugation available upon request

Product Specific Information

The H57-597 monoclonal antibody reacts with the beta chain of mouse TCR. TCR beta is expressed by thymocytes in a developmentally regulated manner and a majority of peripheral T cells. Crosslinking of the TCR complex with H57-597 induces activation and proliferation of T cells or apoptosis depending on assay conditions. It serves as a phenotypic marker for TCR beta expressing T cells.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse thymocytes and splenocytes
  • Recommended use: ≤ 0.5 µg per test (100 µL final volume)
  • Cell number: 10⁵–10⁸ cells/test (empirically determined)
  • Optimize titration for best assay performance
• Excitation / Emission: 488 nm / 520 nm (Blue laser)
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

T cell receptors (TCRs) are essential for distinguishing foreign from self-peptides presented by MHC class II molecules, playing a key role in adaptive immunity. Recognition of self-peptides is linked to autoimmune diseases and tumor-associated mutations. Superantigen engagement of TCRs can lead to toxic shock syndrome. Autoantibodies to V beta segments have been found in RA and SLE patients, potentially modulating TH1-mediated inflammation.

Human T cells mainly express TCR alpha-beta with CD4 or CD8. A subset lacking both (double negative) is elevated in certain autoimmune and immunodeficiency disorders. Gamma-delta T cells, found in epithelial tissues, show distinct antigen recognition and immunoregulatory or antitumor functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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