Thermo Fisher Scientific TCR beta Monoclonal Antibody (H57-597), FITC, eBioscience
마우스 TCR 베타 체인을 인식하는 H57-597 단클론 항체로, FITC 형광 표지되어 있습니다. Flow cytometry에 최적화되어 있으며, 0.5 µg/test로 사용 가능합니다. PBS 버퍼에 0.09% sodium azide 함유, 4°C 암소 저장 권장.
- 카탈로그번호
- 11-5961-8x (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific TCR beta Monoclonal Antibody (H57-597), FITC, eBioscience
Thermo Fisher Scientific TCR beta Monoclonal Antibody (H57-597), FITC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 83 publications |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | H57-597 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465322 |
Available Formats
- Unconjugated
- Alexa Fluor 532
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 496
- Brilliant UV 661
- Brilliant UV 737
- Brilliant Violet 421
- eFluor 450
- eFluor 506
- Functional Grade
- NovaFluor Blue 510
- NovaFluor Blue 530
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Blue 725
- NovaFluor Blue 800
- NovaFluor UV 765
- NovaFluor Violet 690
- NovaFluor Violet 800
- NovaFluor Yellow 810
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Super Bright 780
- Custom conjugation available upon request
Product Specific Information
The H57-597 monoclonal antibody reacts with the beta chain of mouse TCR. TCR beta is expressed by thymocytes in a developmentally regulated manner and a majority of peripheral T cells. Crosslinking of the TCR complex with H57-597 induces activation and proliferation of T cells or apoptosis depending on assay conditions. It serves as a phenotypic marker for TCR beta expressing T cells.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Tested on mouse thymocytes and splenocytes
- Recommended use: ≤ 0.5 µg per test (100 µL final volume)
- Cell number: 10⁵–10⁸ cells/test (empirically determined)
- Optimize titration for best assay performance
- Excitation / Emission: 488 nm / 520 nm (Blue laser)
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
T cell receptors (TCRs) are essential for distinguishing foreign from self-peptides presented by MHC class II molecules, playing a key role in adaptive immunity. Recognition of self-peptides is linked to autoimmune diseases and tumor-associated mutations. Superantigen engagement of TCRs can lead to toxic shock syndrome. Autoantibodies to V beta segments have been found in RA and SLE patients, potentially modulating TH1-mediated inflammation.
Human T cells mainly express TCR alpha-beta with CD4 or CD8. A subset lacking both (double negative) is elevated in certain autoimmune and immunodeficiency disorders. Gamma-delta T cells, found in epithelial tissues, show distinct antigen recognition and immunoregulatory or antitumor functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.