Thermo Fisher Scientific NK1.1 Monoclonal Antibody (PK136), FITC, eBioscience
FITC 결합된 PK136 클론의 NK1.1 단클론 항체로, 마우스 NK 및 일부 T 세포 표지에 사용. Flow cytometry에 최적화되어 있으며 488/520 nm 형광 특성 보유. 4°C 암소 보관, 다양한 형광 형식 제공.
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Thermo Fisher Scientific NK1.1 Monoclonal Antibody (PK136), FITC, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 2 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | View 96 publications |
| Miscellaneous PubMed (Misc) | - | View 2 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PK136 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465317 |
Available Formats
- Unconjugated
- Alexa Fluor 561, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395–805 series
- Brilliant Violet 421–786 series
- eFluor 450
- Functional Grade
- PE, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436–780 series
- Custom conjugation available
Product Specific Information
The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer cells and a subset of T cells in NK1.1 mouse strains including C57BL and NZB. Strains such as BALB/c, SJL, AKR, CBA, C3H, and A do not express NK1.1 antigen. For detection of NK cells in these strains, use monoclonal antibody DX5 (14-5971).
Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 shows coexpression of both markers by most cells and small populations of DX5⁺PK136⁻ and DX5⁻PK136⁺ cells.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of C57Bl/6 mouse splenocytes; ≤0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test).
Careful titration recommended for optimal performance.
Excitation: 488 nm Emission: 520 nm Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
NK1.1 (KLRB1, NKR-P1A, CD161) is expressed on Natural Killer (NK) cells and some T lymphocytes. It mediates cytotoxicity and cytokine secretion upon immune stimulation. The KLRB1 protein, a type II membrane protein, contains a C-type lectin domain, transmembrane region, and cytoplasmic domain. Its receptor NKR-P1A recognizes Lectin Like Transcript-1 (LLT1) as a functional ligand.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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