Thermo Fisher Scientific MHC Class I (H-2Kb) Monoclonal Antibody (AF6-88.5.5.3), FITC, eBioscience
FITC로 표지된 AF6-88.5.5.3 클론의 Mouse MHC Class I (H-2Kb) 단클론 항체. Flow cytometry 및 ICC/IF에 사용 가능. 항원제시 관련 연구에 적합하며, 488 nm/520 nm 형광 특성. 4°C 암소 보관, 동결 금지.
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Thermo Fisher Scientific MHC Class I (H-2Kb) Monoclonal Antibody (AF6-88.5.5.3), FITC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 1 µg/test | View 19 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AF6-88.5.5.3 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11151335 |
Available Formats
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- eFluor 450
- NovaFluor Blue 510
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Red 700
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 702
- Request custom conjugation
Product Specific Information
This AF6-88.5.5.3 monoclonal antibody reacts with the H-2Kb MHC class I alloantigen.
H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8.
Reactivity to other haplotypes (e.g., d, f, j, k, p, q, r, s, u, and v) has not been observed.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes.
Recommended usage ≤ 1 µg/test (100 µL final volume, 10⁵–10⁸ cells/test).
Careful titration is recommended for optimal assay performance.
Fluorescence Properties:
- Excitation: 488 nm
- Emission: 520 nm
- Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
MHC class I alloantigen involved in antigen presentation to CD3/TCR and CD8 T cells.
HLA and MHC antibodies are key tools in Immunopeptidomics, aiding identification and characterization of neoantigens via LC-MS/MS.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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