Thermo Fisher Scientific Bax Chimeric Recombinant Rabbit Monoclonal Antibody (6A7)
Bax 단백질의 활성형 형태를 선택적으로 인식하는 키메라 재조합 토끼 단클론 항체. Western blot, IHC, IP 등 다양한 응용 가능. 인간, 마우스, 랫트 반응성. Protein A 정제, 1 mg/mL 농도, PBS 완충액에 ProClin 300 포함. 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 3 µg/mL |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 6A7 |
| Immunogen | KLH conjugated to peptides corresponding to amino acids 3–16 of murine Bax (CGSGEQLGSGGPTSS), amino acids 3–16 of human Bax (CGSGEQPRGGGPTSS), and amino acids 12–24 shared by Bax from both species (CGPTSSEQIMKTGA) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_3074216 |
Product Specific Information
Specificity:
In the presence of nonionic detergent, the antibody avidly binds the monomeric form of Bax, but not Bax complexed with either Bcl-XL or Bcl-2. Bax antibody only recognizes conformationally changed active Bax.
Preparation:
KLH conjugated to peptides corresponding to amino acids 3–16 of murine Bax (CGSGEQLGSGGPTSS), amino acids 3–16 of human Bax (CGSGEQPRGGGPTSS), and amino acids 12–24 shared by Bax from both species (CGPTSSEQIMKTGA).
Target Information
BAX is a member of the Bcl-2 family and plays an important role in the regulation of apoptosis. Whereas Bcl-2 is commonly regarded as an anti-apoptotic protein, BAX has a pro-apoptotic function. Regulation of apoptosis involves both homo- and heterodimerization of different isoforms of BAX and Bcl-2.
The Bax gene encodes different isoforms including Bax alpha (21 kDa) and Bax beta (24 kDa). Both isoforms contain the BH1, BH2, and BH3 domains; however, Bax beta has a unique carboxyl terminus and lacks a hydrophobic transmembrane domain. Bcl-2 also exists in multiple isoforms, with Bcl-2 beta differing in the 3′ UTR and coding region compared to variant alpha.
Overexpression of BAX promotes cell death through dimerization and interaction with other BCL-2 family proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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