Thermo Fisher Scientific Bax Chimeric Recombinant Rabbit Monoclonal Antibody (6A7)

Applications and Tested Dilution

Product Specifications

Product Specific Information

Specificity:
In the presence of nonionic detergent, the antibody avidly binds the monomeric form of Bax, but not Bax complexed with either Bcl-XL or Bcl-2. Bax antibody only recognizes conformationally changed active Bax.

Preparation:
KLH conjugated to peptides corresponding to amino acids 3–16 of murine Bax (CGSGEQLGSGGPTSS), amino acids 3–16 of human Bax (CGSGEQPRGGGPTSS), and amino acids 12–24 shared by Bax from both species (CGPTSSEQIMKTGA).

Target Information

BAX is a member of the Bcl-2 family and plays an important role in the regulation of apoptosis. Whereas Bcl-2 is commonly regarded as an anti-apoptotic protein, BAX has a pro-apoptotic function. Regulation of apoptosis involves both homo- and heterodimerization of different isoforms of BAX and Bcl-2.

The Bax gene encodes different isoforms including Bax alpha (21 kDa) and Bax beta (24 kDa). Both isoforms contain the BH1, BH2, and BH3 domains; however, Bax beta has a unique carboxyl terminus and lacks a hydrophobic transmembrane domain. Bcl-2 also exists in multiple isoforms, with Bcl-2 beta differing in the 3′ UTR and coding region compared to variant alpha.

Overexpression of BAX promotes cell death through dimerization and interaction with other BCL-2 family proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.