Thermo Fisher Scientific CD314 (NKG2D) Monoclonal Antibody (A10), Functional Grade, eBioscience

Thermo Fisher Scientific CD314 (NKG2D) Monoclonal Antibody (A10), Functional Grade

Applications and Tested Dilutions

Product Specifications

Additional Formats

• Biotin conjugate
• Request custom conjugation

Product Specific Information

The A10 monoclonal antibody reacts with mouse NKG2D, a lectin-like molecule expressed on NK cells. Mouse NKG2D binds to RAE-1 (alpha–epsilon) and H60 molecules, costimulating multiple NK activation receptors via DAP12/DAP10 adaptors. It is expressed by spleen and liver NK cells, NK1.1(+) thymocytes, activated LAK cells, and splenic NKT subsets.

A10 and C7 antibodies detect NK cells from all inbred mouse strains. In redirected lysis assays, A10 stimulates NK cells to kill Daudi targets and induces GM-CSF production via CD16 engagement. A10 and C7 compete for binding to transfected cells, suggesting overlapping epitopes. While C7 is neutralizing, A10 is activating.

Expression of NKG2D on mouse peripheral NK and NKT cells can be detected by flow cytometry using mAb CX5 with higher intensity.

Applications Reported: Flow cytometry and functional activation assays.
Applications Tested: Flow cytometric analysis of mouse splenocytes at ≤1 µg/test (100 µL final volume, 10^5–10^8 cells/test). Titrate for optimal performance.

Storage and Handling: Use in sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: >90% (SDS-PAGE).
Endotoxin Level: <0.001 ng/µg antibody (LAL assay).
Aggregation: <10% (HPLC).

Target Information

Natural killer (NK) cells mediate lysis of tumor and virus-infected cells without prior activation. They regulate humoral and cell-mediated immunity and express calcium-dependent (C-type) lectins involved in NK function. The NKG2 gene family encodes type II transmembrane proteins with C-type lectin domains, located within the NK complex region.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.