Thermo Fisher Scientific ROCK1 Polyclonal Antibody

Applications

• Western Blot (WB): 1:500–1:3,000 dilution tested

Product Specifications

Product Specific Information

• Recommended positive control: HeLa cells
• Predicted reactivity: Mouse (100%), Rat (100%), Rabbit (100%), Rhesus Monkey (100%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

ROCK1 is a serine/threonine kinase that regulates cell differentiation and migration. The Rho/ROCK pathway is involved in the progression of various human cancers. ROCK1 has been identified as a Caspase-3 substrate.
ROCK, an effector of the small GTPase Rho, contributes to myosin light chain (MLC) activation through two pathways: direct phosphorylation of MLC and phosphorylation/inhibition of MLC phosphatase. This leads to increased cellular contractility necessary for apoptotic membrane blebbing.
ROCK1 consists of an amino-terminal kinase domain and a cysteine/histidine-rich C-terminal inhibitory domain within a pleckstrin-homology region, connected by a variable region containing the Rho-binding domain. During apoptosis, caspase-3 cleaves ROCK1 at a conserved DETD1113/G sequence, removing its inhibitory domain and resulting in constitutive kinase activity. This cleavage induces MLC phosphorylation and membrane blebbing, an early event in apoptosis execution.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.