
Thermo Fisher Scientific IKAROS Polyclonal Antibody
IKAROS 단백질을 표적하는 Rabbit Polyclonal Antibody로, WB 및 IHC(P) 실험에 적합. N-말단 합성 펩타이드 기반 면역원 사용. 열매개 항원 회수 권장. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from near N-terminus of human IKAROS protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2549920 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 1 mM EDTA buffer, pH 8.0, for 10 minutes followed by cooling at room temperature for 20 minutes.
Following antigen retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
A suggested positive control is tonsil tissue.
Target Information
DNA-binding protein IKAROS functions in the specification and maturation of T lymphocytes.
It interacts with a critical control element in the terminal deoxynucleotidyl transferase promoter and with promoters for other genes expressed during early stages of B-cell and T-cell development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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