Thermo Fisher Scientific Cytokeratin 14 Polyclonal Antibody
Cytokeratin 14을 인식하는 Rabbit Polyclonal Antibody로, IHC 및 IHC(P) 실험에 적합합니다. 포르말린 고정 조직 염색 시 Pepsin 전처리 필요. 피부 또는 편평세포암을 양성 대조군으로 권장하며, 연구용으로만 사용됩니다.
- 카탈로그번호
- PA532460
- 판매단위
- pk
카탈로그
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from C-terminus of human Keratin 14 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.235 mg/mL |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2549929 |
Product Specific Information
Staining of formalin-fixed tissues requires digesting tissue sections with Pepsin for 10 minutes at 37°C, followed by incubation with primary antibody for 10 minutes at room temperature.
A suggested positive control is skin or squamous cell carcinoma.
Target Information
Cytokeratins (CK) are intermediate filaments of epithelial cells, found in both keratinizing tissues (e.g., skin) and non-keratinizing cells (e.g., mesothelial cells).
At least 20 different CKs can be identified, which are classified into two groups:
- Type I (acidic polypeptides)
- Type II (basic polypeptides)
Keratin 14, a type I low molecular weight keratin, pairs with keratin 5 and serves as a marker distinguishing stratified epithelial cells from simple epithelial cells.
It is useful in identifying squamous cell carcinomas and is considered a prognostic marker in breast carcinomas.
Both acidic and basic keratin types are expressed in all epithelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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