Thermo Fisher Scientific SMURF2 Monoclonal Antibody (OTI3E4), TrueMAB
SMURF2 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Western blot 및 IHC(P) 실험에 적합합니다. 인간 유래 재조합 단백질(aa 151-440)을 면역원으로 제작되었으며, carrier-free 형태로 제공되어 결합 실험에도 활용 가능합니다. 연구용으로만 사용됩니다.
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Applications
- Western Blot (WB): 1:2,000
- Immunohistochemistry (Paraffin) (IHC (P)): 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3E4 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 151–440 of human SMURF2 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
SMURF2 is an E3 ubiquitin-protein ligase that accepts ubiquitin from an E2 ubiquitin-conjugating enzyme and transfers it to target substrates.
It interacts with SMAD1, SMAD2, and SMAD7 to induce their ubiquitination and proteasome-dependent degradation.
SMURF2 enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2.
Coexpression with SMAD1 decreases SMAD1 protein levels and slightly reduces SMAD2 levels.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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