Thermo Fisher Scientific SMURF2 Monoclonal Antibody (OTI6E12), TrueMAB
SMURF2 단백질을 표적으로 하는 Mouse Monoclonal Antibody로, Western Blot에 최적화되어 있습니다. Human에 반응하며, Lyophilized 형태로 제공됩니다. 고순도의 Affinity chromatography 정제 제품으로 연구용에 적합합니다.
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Applications
- Western Blot (WB): Tested dilution 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI6E12 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 151–440 of human SMURF2 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
SMURF2 is an E3 ubiquitin-protein ligase that transfers ubiquitin from an E2 conjugating enzyme to specific substrates. It interacts with SMAD1, SMAD2, and SMAD7, promoting their ubiquitination and proteasome-dependent degradation. SMURF2 enhances SMAD7 inhibitory activity and reduces SMAD2 transcriptional activity. Coexpression with SMAD1 decreases SMAD1 protein levels and moderately reduces SMAD2 levels.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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