Thermo Fisher Scientific SMURF2 Monoclonal Antibody (OTI5E4), TrueMAB
SMURF2 단백질을 표적으로 하는 인간 반응성 마우스 단클론 항체. Western blot에 최적화되어 있으며, 무보존제·동결건조 형태로 제공. 재구성 및 결합 실험에 적합한 고순도 TrueMAB 항체.
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Applications
- Western Blot (WB)
Tested Dilution: 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5E4 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 151–440 of human SMURF2 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to reach a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
SMURF2 is an E3 ubiquitin-protein ligase that transfers ubiquitin from an E2 conjugating enzyme to substrate proteins. It interacts with SMAD1, SMAD2, and SMAD7 to promote their ubiquitination and degradation via the proteasome. SMURF2 enhances the inhibitory activity of SMAD7 and reduces SMAD2 transcriptional activity. Coexpression with SMAD1 decreases SMAD1 protein levels significantly and slightly reduces SMAD2 levels.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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