Thermo Fisher Scientific Adipolean (gAcrp30) Polyclonal Antibody, Biotin, PeproTech
Biotin으로 표지된 Goat Polyclonal Anti-Human Adipolean 항체로, ELISA 및 Western Blot에 적합. 고순도 재조합 단백질로 면역화 후 친화 크로마토그래피로 정제. 인슐린 저항성 및 지방 대사 연구용으로 활용 가능.
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Thermo Fisher Scientific Adipolean (gAcrp30) Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.25–1.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Goat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human gAcrp30/Adipolean |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
Product Specific Information
AA Sequence of recombinant protein:
MKGEPGEGAY VYRSAFSVGL ETYVTIPNMP IRFTKIFYNQ QNHYDGSTGK FHCNIPGLYY FAYHITVYMK DVKVSLFKKD KAMLFTYDQY QENNVDQASG SVLLHLEVGD QVWLQVYGEG ERNGLYADND NDSTFTGFLL YHDTN
Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human gAcrp30/Adipolean. Anti-Human gAcrp30/Adipolean-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
To detect hAdipolean by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Adipolean (500-P193G) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hAdipolean.
Western Blot:
To detect Human Adipolean by Western Blot analysis, this antibody can be used at 0.1–0.2 µg/mL. Used with compatible secondary reagents, detection limit for Recombinant Human Adipolean is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Packaging:
500-P193GBT-1MG is provided as 2 × 500 µg.
Target Information
gAcrp30 is a naturally occurring globular protein obtained by proteolytic processing of adiponectin. Adiponectin is produced and secreted exclusively by adipocytes and is a relatively abundant plasma protein, accounting for up to 0.05% of total serum protein.
Like adiponectin, gAcrp30 decreases hyperglycemia and reverses insulin resistance. It also promotes fat loss by signaling muscle to absorb and burn free fatty acids (FFAs).
The receptors for adiponectin and gAcrp30, AdipoR1 and AdipoR2, have been identified, with AdipoR2 predominantly expressed in the liver.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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