Thermo Fisher Scientific Adipolean (gAcrp30) Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific Adipolean (gAcrp30) Polyclonal Antibody, Biotin

Applications and Tested Dilutions

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MKGEPGEGAY VYRSAFSVGL ETYVTIPNMP IRFTKIFYNQ QNHYDGSTGK FHCNIPGLYY FAYHITVYMK DVKVSLFKKD KAMLFTYDQY QENNVDQASG SVLLHLEVGD QVWLQVYGEG ERNGLYADND NDSTFTGFLL YHDTN

Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human gAcrp30/Adipolean. Anti-Human gAcrp30/Adipolean-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hAdipolean by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Adipolean (500-P193G) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant hAdipolean.

Western Blot:
To detect Human Adipolean by Western Blot analysis, use at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human Adipolean is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Packaging:
500-P193GBT-1MG will be provided as 2 × 500 µg.

Target Information

gAcrp30 is a naturally occurring globular protein obtained by proteolytic processing of adiponectin. Adiponectin is produced and secreted exclusively by adipocytes and is a relatively abundant plasma protein, accounting for up to 0.05% of total serum protein. Like adiponectin, gAcrp30 decreases hyperglycemia and reverses insulin resistance. It also promotes fat loss by signaling muscle to absorb and burn free-fatty acids (FFAs). The signaling receptors for adiponectin and gAcrp30, AdipoR1 and AdipoR2, have been identified, with AdipoR2 predominantly expressed in the liver.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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